QSense QSX 340 His-tag Capturing Sensor
Immobilizing molecules on a surface is a successful approach for label-free studies of biomolecular interactions. Q-Sense His-tag Capturing Sensor, QSX 340, enables immobilization of His-tagged recombinant proteins. The His-residues display a high-affinity for the Cu2+ ions on the sensor surface.
- His-tagged molecule of interest
- Maximum access to protein interaction site by controlled steric orientation
- Surface regeneration possible
- Usage include antibody optimization, protein-protein interactions and probing of conformational changes
Recombinant proteins are often synthesized with an N-terminal or C-terminal hexa-histidine tag (His-tag) to aid in purification from the cell lysate. The His-tag can be used as a convenient and robust way of immobilizing the protein of interest in an oriented and functional manner on a sensor surface.
|Typically ships within||8 weeks|
|Surface Chemistry||Poly (ethylene glycol) (PEG) 3 nm coating and functionalization with a 1013 – 1014/cm2 density of Cu2+ ions.|
|Binding||The Poly-histidine tag displays a high affinity towards M2+ ions such as Cu2+. The binding can be reversed by injection of EDTA or Imidazole.|
|Specificity||The zero-background PEG coating eliminates non-specific binding.|
|Usage||Direct mounting into the instrument from the box without prior cleaning.|
|Storage||Stable > 8 weeks from package date when stored in original vacuum-bag at -20 to -80 °C. Opened sensors should be stored in vacuum desiccator or in 100% nitrogen environment.|
Note: QSensors are developed and produced to provide you with stable, reliable and reproducible data. Full performance is ensured through extensive quality controls, and guaranteed for one-time use according to the recommendations.